The Rapid Translation System (RTS) is an “open” cell-free protein expression system, in which reaction conditions can easily be adapted in a protein-specific manner by adding co-factors, ligands, detergents, or chaperones to the reaction mixture.

The biotechrabbit RTS GroE Supplement provides the GroEL and GroES (together referred to as GroE), which are cytoplasmic chaperones of E. coli reported to be involved in the de novo folding of 10–30 % of all cytoplasmic proteins. It is not possible to predict that a particular protein is a substrate for GroE, GroEL or GroES.
However, the presence of GroE during cellular or cell-free protein synthesis has been reported to lead to higher amounts of correctly folded and active product in many cases. The RTS GroE Supplement can be used with the RTS E. coli HY platform.

(former product codes: RiNA N-1602-05, 5PRIME 2401030)

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

• Component testing: each component is tested to ensure the composition and quality meet stated specifications.
• Performance testing: each product is tested to ensure it meets the stated performance specification.

The RTS GroE supplement can be used to enhance solubility and the functional activity of proteins expressed in the RTS. As an example, the enzyme Rhodanese (35 kDa) was expressed in the presence or absence of RTS GroE supplement using the RTS 500 E. coli HY Kit. The effect of the supplement on solubility and functionality was analyzed by SDS-PAGE and an enzymatic activity assay.

For detailed protocol, please, refer to the product manual (Related Documents).

Product manual

Other documentation