The biotechrabbit RTS 100 E. coli HY Kit contains a modified high-yield E. coli lysate to drive coupled in vitro transcription and translation reactions. The enhanced kit can express up to 20 μg protein (50 μl reaction) in 4 hours using a batch mode. The RTS 100 E. coli HY kit is compatible with both linear templates and plasmids, making it the product of choice for protein-expression screening and optimization.

Each kit contains everything you need to perform protein-expression in the delivered microplate or, alternatively, in microtubes.

(former product codes: 24 rxn kit RiNA N-1101-24, 5PRIME 2401100 / 96 rxn kit RiNA N-1101-96, 5PRIME 2401110)

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

• Component testing: each component is tested to ensure the composition and quality meet stated specifications.
• Performance testing: each product is tested to ensure it meets the stated performance specification.

The RTS 100 E. coli HY Kit is designed to turn genes into proteins within several hours, producing microgram yields to facilitate the assignment of biological functions to genes. Protein expression conditions can be optimized to enhance active protein production by adding chaperones, detergents, or other reagents to the reaction. The microplate format is easily adapted to high-throughput protein expression.

For detailed protocol, please, refer to the product manual (Related Documents).

Product manual

pIVEX2.3-GFP sequence

Other documentation