RPA
biotechrabbit RPA Products
What is Recombinase Polymerase Amplification (RPA)?
Recombinase Polymerase Amplification (RPA) is an isothermal DNA and RNA amplification technology. Unlike traditional PCR methods, RPA eliminates the need for thermal cycling, making it faster and more accessible for both field and laboratory applications.
Key Features
- Isothermal Amplification: Reaction at a constant temperature (37–42°C), no need for thermocyclers.
- Rapid Results: Amplification can be achieved in as little as 10–20 minutes.
- High Sensitivity: Detects as few as 100 DNA or RNA copies.
- Versatile Applications: Suitable for research, diagnostics, and point-of-care testing.
Comparison: RPA vs. PCR
| Feature | RPA | PCR |
|---|---|---|
| Temperature | Isothermal (37–42°C) | Thermal cycling (typically 95°C, 60°C, 72°C) |
| Speed | Results in 10–20 minutes | 1–2 hours |
| Equipment | Minimal (e.g., heating block) | Requires thermal cycler |
| Portability | Ideal for field testing | Limited portability |
| Sensitivity | High sensitivity, few DNA/RNA copies needed | High, but often requires clean samples |
| Applications | Point-of-care, diagnostics, field work | Lab-based diagnostics and research |
Single Enzymes for RPA
Lyo-ready T6 UvsX DNA Recombinase, 5 mg/ml
LYO-ready T4 UvsY Protein, 2 mg/ml
LYO-ready RB69 Gene32 Protein, 10 mg/ml
LYO-ready Exonuclease III, 100 U/µl
RPA Master Mixes
| Product Code | Name | Quantity |
|---|---|---|
| BC1600100 | 2X RPA Basic Master Mix, lyophilized | 8 reactions |
| BC1600101 | 2X RPA Basic Master Mix, lyophilized | 96 reactions |
| BC1600200 | 2X RPA Exo Master Mix, lyophilized | 8 reactions |
| BC1600201 | 2X RPA Exo Master Mix, lyophilized | 96 reactions |
| BC1600300 | 2X RT-RPA Basic Master Mix, lyophilized | 8 reactions |
| BC1600301 | 2X RT-RPA Basic Master Mix, lyophilized | 96 reactions |
| BC1600400 | 2X RT-RPA exo Master Mix, lyophilized | 8 reactions |
| BC1600401 | 2X RT-RPA exo Master Mix, lyophilized | 96 reactions |
Exo vs. Non-Exo Mixes
Exo Master Mixes
- Enable real-time fluorescence detection for quantitative analysis.
- Ideal for sensitive applications like pathogen load monitoring.
Non-Exo (Basic) Master Mixes
- Designed for end-point detection via colorimetric or gel-based methods.
- Cost-effective for simpler workflows.
