biotechrabbit™ Proteinase K is an active endopeptidase that is effective with native proteins, allowing endogenous RNases and DNases to be inactivated rapidly. The robust enzyme is stable over a wide pH range (4–12.5) and remains fully active for several hours when incubated at pH 6.5–9.5. The exceptional purity of the enzyme ensures that it is ideally suited for preparing PCR templates, as it is free of RNases, DNases and DNA.

biotechrabbit Proteinase K is available as an aqueous solution or lyophilized powder.

Storage

Store lyophilised powder at 4°C or room temperature. Store solution at 4°C.

Quality Control

Protein Concentration

The protein concentration of soluble Proteinase K is analyzed by Biuret assay (20 mg, +/- 2 mg).

Protease activity

The activity is analyzed with hemoglobin as substrate at 37°C (>600 U/ml).

Absence of nuclease activity

The enzyme is tested for absence of endonuclease and nicking activities.

RNase contamination

The enzyme is tested for absence of RNase activity.

DNA contamination

The enzyme is tested for DNA (threshold 10 pg/mg).

Bioburden

The enzyme is tested for biodurden (<1 CFU/ml).

Useful Hints

Inhibitors
The enzyme is not inactivated by metal ions, chelating agents (such as EDTA), sulfhydryl reagents, or trypsin and chymotrypsin inhibitors.

Activators
The Proteinase K activity is stimulated by denaturing agents (SDS and urea). The presence of SDS can produce a seven-fold increase in Proteinase K activity.

pH Optimum
Proteinase K is stable over a broad pH range (4 – 12.5). The enzyme retains its full activity for several hours when incubated at a pH between 6.5 and 9.5.

Product manual Proteinase K lyophilized

Product manual Proteinase K soluble

Other documentation