His-tag Protein Binding
biotechrabbit is holding the global license from Qiagen to manufacture and sell Tris-NTA.
Modern proteomics relies on reliable and sensitive detection and labeling of tagged protein, such as those bearing the 6xHis-tag. However, the low affinity of currently available chelators used for protein binding limits the sensitivity of many applications for selective, site-specific conjugation of proteins. To address this issue, biotechrabbit offers a superior His-tag protein ligand for the reversible binding of proteins and cell surfaces: Tris-NTA.
With a binding affinity which is approximately four orders of magnitude higher than monovalent metal ion chelators (such as nitrilotriacetic acid and iminodiacetic acid), Tris-NTA increases the sensitivity in a western hybridization, biosensor analysis, functional protein analysis, protein purification and many other applications.
Extremely high sensitivity — 10,000 times higher binding affinity than conventional chelators
Diverse application — for reversible binding of proteins, oligosaccharides, fluorophores, lipids, etc.
Minimal effects on protein function — for greater flexibility in functional assays
-
Tris-NTA Amine
Features
- A complex of three Ni-NTA groups ensures high-affinity binding of His-tags
- Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
- Protein binding is stoichiometric
Learn More…Applications
- Reversible labeling of proteins or cell surfaces
- Detection and analysis of target molecules
- Immobilization of proteins, lipids and cells on surfaces
- Purification and sample preparation of proteins
- Coupling with microscopic or spectroscopic probes
Package Sizes
Starting at: €215.00Does not include applicable tax, shipping & handling, or other charges.
-
Tris-NTA Biotin
Features
- A complex of three Ni-NTA groups ensures high-affinity binding of His-tags
- Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
- Protein binding is stoichiometric
Learn More…Applications
- Reversible labeling of proteins or cell surfaces
- Detection and analysis of target molecules
- Immobilization of proteins, lipids and cells on surfaces
- Purification and sample preparation of proteins
- Coupling with microscopic or spectroscopic probes
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
