STARTING MATERIAL

Fresh, frozen or dried plant tissue (maximum 100 mg dry weight
or 180 mg wet weight)

EXTRACTION TIME

Approximately 40 min

BINDING CAPACITY

> 50 µg DNA

TYPICAL YIELD

Variable depending on the starting material; approximately 5–25 µg DNA

AVERAGE PURITY

A₂₆₀/A₂₈₀ 1.7–2.0

STEPS

• Homogenize plant material.
• Mortar and pestle in presence of liquid nitrogen.
• Homogenizer, e.g., rotor-stator or bead mill.
• Lysis dependent on Buffer LYSIS:
  • add Buffer LYSIS LC, incubate and proceed with the next step.
  • add Buffer LYSIS LS or Buffer LYSIS LD, incubate, add Buffer PRECIPITATION and centrifuge to remove unlysed material.

• Filter lysate using a Pre Filter (lavender).

• Bind DNA to the Mini Filter (green) with Buffer BINDING BD.
• Centrifuge to remove unbound material.

• Wash the DNA twice with Buffer WASH C.
• Centrifuge twice to remove the wash buffer.

• Elute DNA with Buffer ELUTION and centrifuge.
• Purified DNA in the Elution Tube is ready for use.