biotechrabbit GenUP™ gDNA Kit is designed for a fast and efficient genomic DNA isolation from various sources and different amounts of starting material, such as mammalian tissues (including paraffin-embedded), buccal swabs and eukaryotic cell cultures. After a few initial procedures, the genomic DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. High yields of pure, unshared genomic DNA are ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
STARTING MATERIAL | Tissue samples (up to 40 mg), rodent tail specimens of 0.5–1 cm in length, eukaryotic cells (up to 5 × 106), formalin-fixed paraffin-embedded tissue samples (FFPE, 2–4 slices), buccal swabs |
EXTRACTION TIME | Approximately 8 min after lysis |
BINDING CAPACITY | > 100 µg DNA |
TYPICAL YIELD | Variable; approximately 65 µg |
AVERAGE PURITY | A260/A280 1.7–2.0 |
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).
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