biotechrabbit™ GenUP Blood DNA Kit is designed for fast isolation of genomic DNA from up to 400 µl whole blood from fresh or frozen samples that have been stabilized with EDTA or citrate. After an efficient lysis step, genomic DNA is bound to a Mini Filter, washed and eluted. The isolation chemistry and extraction protocol are optimized for maximum yield. Including lysis, isolated DNA is available in approximately 24 min. The isolated DNA is suitable for a wide range of different molecular biology applications.
Protocols are available for isolating DNA from 200 µl or 400 µl whole blood samples.
The GenUP Blood DNA Kit is designed for the use with blood. For other starting material, such as cell-free body fluids (including cerebrospinal fluid, serum, plasma or urine), tissue, stool samples, buffy coat, cultured or isolated cells, swabs, dried blood spots, viruses, fungi, bacteria or parasites, please refer to the GenUP gDNA Kit (cat. no. BR0700601), GenUP Bacteria gDNA Kit (cat. no. BR0700701), GenUP Plant DNA Kit (cat. no. BR0700801) or GenUP Virus DNA/RNA Kit (cat. no. BR0701101).
STARTING MATERIAL | Fresh or frozen whole blood; stabilized with EDTA or citrate (200 µl or 400 µl) |
EXTRACTION TIME | Approximately 24 min |
BINDING CAPACITY | > 60 µg DNA |
TYPICAL YIELD | Variable depending on the starting material; approximately 30 µg DNA |
AVERAGE PURITY | A260/A280 1.7–2.0 |
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For detailed protocol, please, refer to the product manual (Related Documents).
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