biotechrabbit™ PCR Enhancer is a unique PCR additive that improves sensitivity, resulting in increased PCR efficiency and reduced background. PCR Enhancer is ideal for difficult templates (e.g., GC-rich) and complex reactions, such as low-abundance templates and multiplex PCR.

Quality Control

Functional Assay

GC-rich DNA was amplified using specific primers to produce a distinct band.

PROTOCOL

The PCR Enhancer lowers the background in complicated PCR with low template amounts, impure templates or high GC content. As a standard, the PCR Enhancer is used in 1× concentration, or can be added to the reaction mixture at the final concentration of 0.5× – 2× according to the table below:

Product manual

Other documentation