biotechrabbit™ PCR Enhancer is a unique PCR additive that improves sensitivity, resulting in increased PCR efficiency and reduced background. PCR Enhancer is ideal for difficult templates (e.g., GC-rich) and complex reactions, such as low-abundance templates and multiplex PCR.
|
Component |
Composition |
|
PCR Enhancer |
Proprietary PCR Enhancer component in water |
|
STORAGE |
-20°C (until expiry date – see product label) |
Quality Control
Functional Assay
GC-rich DNA was amplified using specific primers to produce a distinct band.
PROTOCOL
The PCR Enhancer lowers the background in complicated PCR with low template amounts, impure templates or high GC content. As a standard, the PCR Enhancer is used in 1× concentration, or can be added to the reaction mixture at the final concentration of 0.5× – 2× according to the table below:
Final concentration of PCR Enhancer in a 50 µl reaction | 0.5× | 1× | 1.5× | 2× |
Volume of 5× PCR Enhancer solution to add, in µl | 5 | 10 | 15 | 20 |
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