Hot-Start PCR
biotechrabbit Hot-start PCR products include highly purified YourTaq™ Hot Start DNA Polymerase which is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Polymerase is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. PCR mixes combine YourTaq, optimized buffer and high-quality dNTPs for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70%) by using 5× PCR Enhancer.
Convenient — master mixes including a direct-load version make your job easier
Specific and sensitive — excellent performance for a broad range of amplicons
Efficient — abundant DNA even from low copy targets
Resistant — direct amplification from crude samples
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Hot-Start Taq DNA Polymerase, 5 U/µl
Features
- High PCR specificity and sensitivity
- Exceptionally pure Hot Start Taq DNA Polymerase for sensitive PCR applications and high yields
- Antibody-based Hot Start for fast polymerase activation
Learn More…Applications
- Hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- RT-PCR and TA cloning
Package Sizes
Starting at: €93.00Does not include applicable tax, shipping & handling, or other charges.
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2X Hot-Start PCR Master Mix
Features
- Highest PCR sensitivity without prolonged reactivation
- Optimized PCR Master Mix for minimal hands-on and fast setup
- Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
Learn More…Applications
- High-specificity and high-throughput hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- TA cloning
Package Sizes
Starting at: €114.00Does not include applicable tax, shipping & handling, or other charges.
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2X Hot-Start PCR Master Mix, lyophilized
Features
- Room-temperature stable enzymes and mixes
- Exceptionally pure Taq DNA Polymerase
- Hot-start for high PCR specificity and sensitivity
- Optimized Master Mix for fast setup
Learn More…Applications
- Ambient shipment and room-temperature storage
- Routine and demanding PCR applications
- PCR amplification up to 3 kb
- TA cloning
Package Sizes
Starting at: €149.00Does not include applicable tax, shipping & handling, or other charges.
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YourTaq™ Hot-Start DNA Polymerase, 5 U/µl
Features
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized buffer composition for increased yield of amplification
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- RT-PCR and TA cloning
Package Sizes
Starting at: €61.00Does not include applicable tax, shipping & handling, or other charges.
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2X YourTaq™ Hot-Start PCR Master Mix
Features
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized Master Mix for increased yield of amplification
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- TA cloning
Package Sizes
Starting at: €114.00Does not include applicable tax, shipping & handling, or other charges.
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2X YourTaq™ Direct-Load Hot-Start PCR Master Mix
Features
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized Master Mix for increased yield of amplification and direct loading on the gel
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- TA cloning
Package Sizes
Starting at: €120.00Does not include applicable tax, shipping & handling, or other charges.
-
2X YourTaq™ Hot-Start PCR Master Mix, lyophilized
Features
- Stable enzyme and mix for ambient shipment and room-temperature storage
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized Master Mix for increased yield of amplification
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- TA cloning
Package Sizes
Starting at: €149.00Does not include applicable tax, shipping & handling, or other charges.
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ApStarTaq™ Hot-Start DNA Polymerase, 5 U/µl
Features
- Aptamer-based hot-start functionality
- No activation steps needed
- High PCR specificity and sensitivity
- Exceptionally pure Taq DNA Polymerase for sensitive PCR applications and high yields
Learn More…Applications
- Fast PCR reactions in endpoint and real-time analysis
- Hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- RT-PCR and TA cloning
Package Sizes
Starting at: €61.00Does not include applicable tax, shipping & handling, or other charges.
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UPstart™ Taq Antibody, 1 mg/ml
Features
- Inhibition of >95% Taq activity at 60°C
- 200 ng UPstart Taq Antibody are blocking 1 U Taq DNA polymerase
- Exceptionally pure— made in cell culture
Learn More…Applications
- Thermolabile inhibition of Taq DNA polymerases
- Convenient hot-start PCR setup at room temperature
- Fast polymerase activation with the first PCR denaturation step
Package Sizes
Starting at: €519.00Does not include applicable tax, shipping & handling, or other charges.
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UPstart™ Plus Taq Antibody, 1 mg/ml
Features
- Inhibition of >95% Taq activity at 60°C
- 200 ng UPstart Plus Taq Antibody are blocking 1 U Taq DNA polymerase
- Exceptionally pure— made in cell culture
Learn More…Applications
- Thermolabile inhibition of Taq DNA polymerases
- Convenient hot-start PCR setup at room temperature
- Fast polymerase activation with the first PCR denaturation step
Package Sizes
Starting at: €549.00Does not include applicable tax, shipping & handling, or other charges.
