The cloning vector RTS pIX3.0 is designed for cloning linear templates generated with RTS Linear Template Plus and Fab kits to subsequently up-scale protein synthesis in E. coli batch systems to the CECF format or to use with RTS insect kits. The vector contains a multiple cloning site compatible with that of the linear templates.

The RTS pIX3.0 Vector enables high-level protein expression using the RTS 100/500/9000 E. coli HY HY kits, E. coli Disulfide and RTS Insect kits. The kit exhibits the same performance as the RTS pIVEX His-tag, 2nd Gen. Vector Set, which is used for classic cloning.

(former FastLane product codes: Vector RiNA T-1103-01)

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

• Component testing: each component is tested to ensure the composition and quality meet stated specifications.
• Performance testing: each product is tested to ensure it meets the stated performance specification.

The RTS pIX3.0 Vector is used for upscaling protein synthesis with RTS E. coli-based and Insect-based kits after expression screening using PCR-generated Linear Templates. These PCR products generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit contain multiple cloning sites that are compatible for cloning into the RTS pIX3.0 Vector.
The resulting expression plasmid is added to an upscaled in vitro transcription/translation reaction mix and incubated. After incubation, the proteins can be purified and analyzed, e.g., by SDS-PAGE or functional assays.

For detailed protocol, please, refer to the product manual (Related Documents).

Product manual

pIX3.0 sequence

Other documentation