The RTS Linear Template Fab Kit is designed for rapid PCR-based generation of templates directly used in the E. coli Fab and Insect kits allowing functional screening of antibody fragments and disulfide proteins and to facilitate the assignment of biological functions to genes. The templates can be generated with optional affinity tag and/ or signal peptide sequences for protein purification and translocation into microsomes.

The RTS Linear Template Fab Kit generates templates with elements to drive efficient coupled in vitro transcription and translation reactions for the synthesis of proteins with intra- and intermolecular disulfide bonds. The kit is the product of choice for functional screening of disulfide proteins and optimization of constructs. One kit contains sufficient volumes of primer solutions to perform 96 standard protein expression reactions with reaction volumes of 25 μl.

(former FastLane product codes: RiNA C-1217-96)

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

• Component testing: each component is tested to ensure the composition and quality meet stated specifications.
• Performance testing: each product is tested to ensure it meets the stated performance specification.

Use the RTS Linear Template Fab Kit (96) for the synthesis of templates encoding Fab, scFv and other disulfide proteins.
 
It is designed to turn open reading frames into expressable templates, which can be directly used in RTS 100 E. coli Fab kits, RTS 100 Insect Disulfide kits and RTS 100 E. coli HY kits.

Optionally provide your templates with affinity tag or signal peptide sequences for protein purification and translocation into microsomes.

For detailed protocol, please, refer to the product manual (Related Documents).

Product manual

Other documentation