Vectors and Linear Template Kits
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RTS Linear Template Kit Plus
Features
- Efficient in vitro expression (E. coli and insect kits)
- Primers for affinity tags (His6-tag /Strep-tag II)
- Signal peptide sequence primers for expression translocation into microsomes (insect systems)
Applications
- Fast generation of multiple template constructs
- Accelerated screening of protein expression templates and conditions
Package Sizes
Starting at: €345.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Linear Template Fab Kit
Features
- Introduction of an affinity tag for protein purification
- Signal peptide sequence for translocation into microsomes
- Introduction of a signal peptide sequence for translocation into microsomes
- Compatibility with E. coli HY, Fab and Insect kits
Applications
- PCR-based inear templates for protein expression in E. coli and insect disulfide kits
- Fast template generation for expression of Fab and scFv antibody fragments
Package Sizes
Starting at: €360.00Does not include applicable tax, shipping & handling, or other charges.
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RTS 100 E. coli LinTempGen Set, His-tag
Features
- Contains all regulatory elements in optimal spacing
- Option for templates with N- or C-terminal His6-tag
- Primary PCR product can be cloned into pIVEX vectors
- For use with RTS 100 E. coli HY and insect kits
Applications
- Fast gene template generation without cloning
- Generate linear templates for rapid screening and optimization of His6-tagged protein expression
Package Sizes
Starting at: €400.00Does not include applicable tax, shipping & handling, or other charges.
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RTS Wheat Germ LinTempGen Set, His6-tag
Features
- Optimized spacing of eukaryotic regulatory elements
- Time saving, no laborious cloning
- Flexible positioning of His6-tag at the C- or N-terminus
- Primary PCR product can be cloned into pIVEX vectors
Applications
- Rapid generation of linear expression constructs for screening applications
- Linear template generation for optimized His6-tagged protein expression
Package Sizes
Starting at: €350.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIX3.0 Vector
Features
- Multiple cloning site compatible with linear templates generated with RTS Linear Template Kit Plus and RTS Linear Template Fab Kit
- High copy origin of replication
- Cell-free protein synthesis scale-up to after expression screening using linear templates
- Free of any potentially interfering regulatory elements ensuring efficient transcription and translation
- Compatible with RTS E. coli and insect kits
Applications
- Cloning of RTS Linear Templates for expression in RTS E. coli and insect kits
Package Sizes
Starting at: €356.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIX4.0 Insect Vector
Features
- Regulatory elements for efficient transcription and translation
- Multiple cloning site and translational stop codons in all reading frames
- Optimized untranslated regions stabilizing and protecting RNA from degradation by exonucleases
- RNA of defined length via optional plasmid linearization
Applications
- Cloning genes for efficient synthesis of proteins using the RTS 100 Insect kits
Package Sizes
Starting at: €356.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIVEX E. coli His-tag, 2nd Gen. Vector Set
Features
- Stable circular templates to ensure high yields in preparative-scale expressions
- For construction of C- and N-terminal His6-tagged proteins
- Optimal spacing of all regulatory elements
- For use with RTS E. coli and Insect kits
Applications
- Preparative-scale expression of His6-tagged proteins
Package Sizes
Starting at: €415.00Does not include applicable tax, shipping & handling, or other charges.
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RTS pIVEX Wheat Germ His6-tag Vector Set
Features
- Stable circular templates ensure high yields in preparative-scale expression reactions
- For construction of C- and N-terminal His6-tag proteins
Applications
- Expression vector for preparative-scale expression in eukaryotic wheat germ lysates
Package Sizes
Starting at: €410.00Does not include applicable tax, shipping & handling, or other charges.