The Rapid Translation System (RTS) is an “open” cell-free protein expression system, in which reaction conditions can easily be adapted in a protein-specific manner by adding co-factors, ligands, detergents, or chaperones to the reaction mixture.

The biotechrabbit RTS DnaK Supplement includes DnaK (heat shock protein 70, hsp 70), one of the key chaperone systems in E. coli, in combination with DnaJ (hsp 40) and GrpE . The RTS DnaK Supplement can be used to increase the amount of correctly folded and/or soluble proteins expressed in the RTS.

DnaK can be used with proteins of any size and serves as an aggregation inhibitor that binds unfolded protein or transfers the protein to other chaperones, such as the GroE or the Hsp90 systems. The RTS DnaK Supplement can be used with the RTS E. coli HY platform.

(former product codes: RiNA N-1601-05, 5PRIME 2401020)

Quality Control

in vitro transcription/translation protein expression products are manufactured using quality chemicals and materials that meet our high standards. All product components are subjected to rigorous quality assurance testing process:

• Component testing: each component is tested to ensure the composition and quality meet stated specifications.
• Performance testing: each product is tested to ensure it meets the stated performance specification.

RTS DnaK Supplement - a tailor-made chaperone mix for optimization of expression in RTS E. coli

The RTS DnaK supplement can be used to enhance solubility and functional activity of proteins expressed with RTS. It contains DnaK, DnaJ and GrpE as components of a lysate produced from an E. coli strain overexpressing these chaperones. Since the action of DnaK depends on ATP/ADP conversion, an ATP-containing Energy Mix is provided as a separate bottle.

An optimized protocol allows easy combination of this lysate with standard reagents from the RTS 100 or 500 E. coli platforms. If initial expression reactions carried out with RTS 100 or 500 lead to misfolded or unsoluble product, the RTS DnaK Supplement offers a useful option to improve the experimental results.

The kit contains enough lysate (5 x 180 µl) to supplement e.g., one hundred RTS 100 reactions.

For detailed protocol, please, refer to the product manual (Related Documents).

Product manual

Other documentation