biotechrabbit | GenUP™ Plasmid Kit

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GenUP™ Plasmid Kit

Features

Universal kit for both high- and low-copy-number plasmids isolation
Fast and simple procedure
High yields of pure plasmid DNA suitable for all applications

Applications

Plasmid DNA isolation from Gram-negative and Gram-positive bacteria

Purchase GenUP™ Plasmid Kit

Availability: In stock

Catalog #	Size	Package Content	Price*
BR0700201	10 preps	Solutions, Mini Filters and Vials	€29.00
BR0700202	50 preps	Solutions, Mini Filters and Vials	€90.00
BR0700203	250 preps	Solutions, Mini Filters and Vials	€369.00

*Does not include applicable tax, shipping & handling, or other charges.

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biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.

The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.

STARTING MATERIAL	Bacterial cultures: 0.5–15 ml for high-copy-number plasmids, low-copy-number plasmids or cosmids
EXTRACTION TIME	Approximately 15 min
BINDING CAPACITY	Approximately 80 µg plasmid DNA
TYPICAL YIELD	Variable: 45–60 µg high-copy plasmid DNA is typically purified from 15 ml culture, or 6–20 µg from 2 ml culture
AVERAGE PURITY	A₂₆₀/A₂₈₀ 1.7–2.0