biotechrabbit | GenUP™ Plant DNA Kit

Return to Previous Page

GenUP™ Plant DNA Kit

Features

Simple and efficient procedure for plant DNA isolation
Special lysis protocols for different plant materials
High yields of inhibitor-free DNA

Applications

Universal kit for isolating genomic DNA from various plant materials

Purchase GenUP™ Plant DNA Kit

Availability: In stock

Catalog #	Size	Package Content	Price*
BR0700801	10 preps	Solutions, Mini Filters and Vials	€54.00
BR0700802	50 preps	Solutions, Mini Filters and Vials	€179.00
BR0700803	250 preps	Solutions, Mini Filters and Vials	€732.00

*Does not include applicable tax, shipping & handling, or other charges.

| Add to Compare
| Email to a Friend

biotechrabbit™ GenUP Plant DNA Kit has been specially developed for quick and easy purification of genomic DNA from a wide variety of plant materials, including fresh, frozen or dried samples from leaves, roots, stems and flowers.

The kit includes an advanced prefiltration step to remove unlysed tissue. Subsequently, DNA is bound to a Mini Filter and is subsequently washed and eluted in a separate tube. The purified DNA is ready for use in any demanding molecular biology application, including PCR, enzymatic digestions and cloning.

This kit provides three buffers for optimized processing with different plant materials. To determine optimal lysis conditions, side-by-side preparation using the three provided protocols are prepared.

STARTING MATERIAL	Fresh, frozen or dried plant tissue (maximum 100 mg dry weight or 180 mg wet weight)
EXTRACTION TIME	Approximately 40 min
BINDING CAPACITY	> 50 µg DNA
TYPICAL YIELD	Variable depending on the starting material; approximately 5–25 µg DNA
AVERAGE PURITY	A₂₆₀/A₂₈₀ 1.7–2.0

STEPS

Homogenize plant material.
Mortar and pestle in presence of liquid nitrogen.
Homogenizer, e.g., rotor-stator or bead mill.
Lysis dependent on Buffer LYSIS:
- add Buffer LYSIS LC, incubate and proceed with the next step.
- add Buffer LYSIS LS or Buffer LYSIS LD, incubate, add Buffer PRECIPITATION and centrifuge to remove unlysed material.