biotechrabbit™ GenUP Exo SAP Kit is designed for quickly and easily cleaning up PCR amplification reactions, enabling their direct use in sequencing or genotyping.
The kit provides a combination of Shrimp Alkaline Phosphatase (SAP) and Exonuclease 1 (ExoI) for removing nucleotides and primers. Double-stranded DNA is not affected, ensuring 100% recovery of PCR products.
Both enzymes are active in PCR buffers and completely inactivated by heating at 80°C: no further processing is required. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, and the 3' → 5' exonuclease activity of Exonuclease 1 degrades single-stranded primers.
Component | Composition |
ExoI-SAP | Exonuclease I and SAP in storage buffer containing 50% glycerol (v/v). |
STORAGE | −20°C (until expiry date – see product label) |
Quality Control
Shrimp Alkaline Phosphatase
Specificity
SAP Removes 5’-phosphates from DNA, RNA, dNTPs and proteins.
Purity
Purified to apparent homogeneity. SAP is highly pure and tested free of contaminating nucleases.
Exonuclease 1
Specificity
ExoI is a 3’-5’ exonuclease, specific for single stranded DNA.
Purity
Purified to apparent homogeneity. ExoI is highly pure and tested free of contaminating nucleases.
STEPS |
|
|
For detailed protocol, please, refer to the product manual (Related Documents).
You may also be interested in the following product(s)
RTS pIVEX Wheat Germ His6-tag Vector Set
Package Sizes |
RTS 100 Wheat Germ CECF Kit
Package Sizes |
GenUP™ Total RNA Kit
Package Sizes |
Tris-NTA Amine
Package Sizes |
5X CAPITAL™ 1-Step qRT-PCR Probe Master Mix, lyophilized
Package Sizes |