biotechrabbit™ deoxynucleotide triphosphates are first-choice nucleotides for all PCR applications, including the most demanding, such as amplification of long targets (up to 40 kb), GC-rich templates, qPCR, cDNA synthesis, high-fidelity PCR, DNA labeling and sequencing.

Advanced production technology ensures that deoxyribonucleotide triphosphates have >99% purity and outstanding stability, ensuring excellent performance and consistent, reliable results.

For the maximum flexibility, nucleotides are available in sets and mixes of common concentrations.

Quality Control

Functional assay

Functionality tested in PCR.

Purity test

The concentration of each lot is verified by optical density spectrometry. The purity of nucleotide triphosphates is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

Contamination test

The absence of human and E. coli DNA is confirmed by qPCR.

Prevention of PCR contamination

When assembling the amplification reactions, care should be taken to eliminate the possibility of contamination with undesired DNA.

• Use separate clean areas for preparation of samples and reaction mixtures and for cycling.
• Wear fresh gloves. Use sterile tubes and pipette tips with aerosol filters for PCR setup.
• Use only water and reagents that are free of DNA and nucleases.
• With every PCR setup, perform a contamination control reaction that does not include template DNA.

Typical use of dNTPs

• 0.2 mM of each nucleotide is the recommended final dNTP concentration in common PCR applications.
• Every time before the use thaw and mix well each dNTP solution, especially highly concentrated ones.
• Use the ready dNTP mixes in PCR (at 0.2 mM final concentration each)

Product manual 25 mM dNTP Mix

Product manual 10 mM dNTP Mix

Product manual 10 mM dN/UTP Mix

Other documentation