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Standard PCR
Standard PCR typically has the following parameters:
- Target length is 1–3 kb
- GC content of the template is 40–60%
- Template is pure, abundant and unproblematic
- Very high-fidelity of amplification is not required
Unmodified Taq DNA polymerase is the most common enzyme for standard PCR. For simplified reaction setup, PCR master mixes which contain all necessary PCR reagents are offered. Green colored, high-density PCR buffer allows direct loading of PCR products onto gels.
More information:
Standard PCR Product Selection Guide
| Product | Main feature | Simplified set-up | Optimization freedom | Direct gel loading | Colored Enzyme |
| Taq DNA Polymerase, 5 u/µl | MgCl2 supplied in a separate vial for maximum flexibility | + | |||
| YourTaq PCR Mix, 2x | Premixed PCR reagents: just add template, primers and water | + | |||
| YourTaq Direct-Load PCR Mix, 2x | Premixed PCR reagents: just add template, primers and water. Green Reaction Buffer for direct loading on gels after the PCR | + | + | + |
NOTE: All above enzymes ensure the same PCR Fidelity like Taq DNA Polymerase, produce PCR products of up to 3 kb
with A overhangs suitable for TA cloning.
