biotechrabbit™ TriColor Broad Protein Ladder facilitates approximate molecular-weight estimation of proteins on denaturing polyacrylamide gels, monitoring protein separation during electrophoresis and verification of western transfer efficiency to membranes (polyvinylidene difluoride, nylon or nitrocellulose). The ladder provides three colored reference bands.
TriColor Broad Protein Ladder is ready for immediate use and is notably stable: sharp bands are produced after storage for 2 weeks at room temperature or 3 months at 4°C, eliminating the need to thaw before loading.
Component | Composition |
TriColor Broad Protein Ladder (3.5–245 kDa) | Ready-to use protein ladder containing 0.1 – 0.4 mg/ml of each protein in 20 mM Tris-phosphate, pH 7.5, 2 % SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol. |
STORAGE | −20°C (until expiry date – see product label) |
Quality Control
Functional assay
Appearance of all stated bands tested in SDS-Polyacrylamide gel electrophoresis.
Component | LOADING VOLUME | ACTIONS BEFORE LOADING |
TriColor Broad Protein Ladder (3.5–245 kDa) | 3 or 5 µl for 15 or 10-well mini-gel, respectively. 1.5 or 2.5 µl for Western transfer. Apply more for thicker (>1.5 mm) or larger gels. | Direct loading |
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