biotechrabbit™ DNA electrophoresis ladders are mixtures of exceptionally purified DNA fragments created either by PCR or by digesting proprietary plasmids with restriction enzymes. Ladders are ready to use and suitable not only for DNA sizing but also for approximate DNA quantification in gels. For convenience, ladders have increased intensity reference bands and indicated DNA amount in nanograms for every band.
Every ready-to-use ladder is supplied with the nuclease-free Loading Dye Solution, which ensures optimal migration and quantification of your DNA probes. It includes three electrophoresis tracking dyes (xylene cyanol, bromophenol blue and orange G), allowing the process of the DNA through the gel to be visualized.
Component | Composition |
1 kb DNA Ladder RTU | Ready-to use DNA in 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, Glycerol and tracking dye (bromophenol blue, xylene cyanol FF) |
DNA Loading Dye | 6× DNA Loading Dye: 10 mM Tris-HCl (pH 8.0), 60 mM EDTA, Glycerol and tracking dyes (bromophenol blue, xylene cyanol FF and orange G) |
STORAGE | −20°C (until expiry date – see product label) |
Quality Control
Functional assay
Tested in gel electrophoresis.
Component | LOADING VOLUME | ACTIONS BEFORE LOADING |
1 kb DNA Ladder | 5 µl | Direct loading |
6× DNA Loading Dye | Add 1 volume of Loading Dye | Mix well |
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