biotechrabbit™ GenUP PCR/Gel Cleanup Kit has been specially developed for a quick and easy cleanup or concentration of PCR fragments from reaction mixtures as well as extraction of DNA from both TAE and TBE agarose gels. The DNA is bound to a Mini Filter using a novel buffer, washed and then eluted in a separate tube. The purified DNA is ready to be used in all demanding molecular biology applications, including restriction digestion, ligation, sequencing, transfection into mammalian cells and in vitro transcription.
STARTING MATERIAL | PCR reaction mixtures (up to 50 µl) | TAE or TBE agarose gel (up to 300 mg) |
EXTRACTION TIME | Approximately 3 min | Approximately 20 min |
BINDING CAPACITY | > 20 µg DNA | > 20 µg DNA |
DNA SIZE | 60 bp – 30 kb | 100 bp – 30 kb |
RECOVERY RATE | 60–95% | 60–90% |
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).
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