biotechrabbit™ GenUP PCR Cleanup Kit has been developed for quick and easy cleanup or concentration of PCR fragments from reaction mixtures. High-yield PCR product with excellent quality is purified from the PCR mixtures in a simple two-step procedure. DNA is bound to a Mini Filter using a novel buffer and eluted. The need for a washing step has been eliminated, reducing hands-on-time. The procedure takes approximately 3 min, compared to 8 min required to other kits. The purified DNA is ready for use in all demanding molecular biology applications, including restriction digestion, ligation and sequencing.
STARTING MATERIAL | Amplification reaction mixtures (up to 50 µl) |
EXTRACTION TIME | Approximately 3 min |
BINDING CAPACITY | > 20 µg DNA |
DNA SIZE | 60 bp – 30 kb |
RECOVERY RATE | 60–95%, depending on length of the PCR fragment |
STEPS |
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For detailed protocol, please, refer to the product manual (Related Documents).
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