biotechrabbit | dNTP Sets - Nucleotides - Products

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dNTP Sets

Features

Exceptional quality dNTPs of >99% purity confirmed by HPLC
Free from DNA and PCR inhibitors
Consistent PCR results due to outstanding dNTPs stability

Applications

Standard or hot-start PCR
Long-range and high-fidelity PCR
cDNA synthesis and RT-PCR
qPCR
Sequencing
DNA labeling

Purchase dNTP Sets

Availability: In stock Bulk requests please inquire at oem@biotechrabbit.com

Catalog #	Size	Package Content	Price*	Qty
BR0600601	4 × 250 µl	250 µl dATP (100 mM solution) 250 µl dCTP (100 mM solution) 250 µl dGTP (100 mM solution) 250 µl dTTP (100 mM solution)	€65.00
BR0600602	4 × 1 ml	1 ml dATP (100 mM solution) 1 ml dCTP (100 mM solution) 1 ml dGTP (100 mM solution) 1 ml dTTP (100 mM solution)	€208.00

*Does not include applicable tax, shipping & handling, or other charges.

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biotechrabbit™ deoxynucleotide triphosphates are first-choice nucleotides for all PCR applications, including the most demanding, such as amplification of long targets (up to 40 kb), GC-rich templates, qPCR, cDNA synthesis, high-fidelity PCR, DNA labeling and sequencing.

Advanced production technology ensures that deoxyribonucleotide triphosphates have >99% purity and outstanding stability, ensuring excellent performance and consistent, reliable results.

For the maximum flexibility, nucleotides are available in sets and mixes of common concentrations.

Component	Composition
all dNTP Sets	Aqueous solution (pH 7.0) containing 2'-deoxy-nucleotide 5'-triphosphate sodium salts

STORAGE

−20°C (until expiry date – see product label)

Quality Control

Functional assay

Functionality tested in PCR.

Purity test

The concentration of each lot is verified by optical density spectrometry. The purity of nucleotide triphosphates is determined by HPLC. The minimum passing specification for the triphosphate content is 99%.

Contamination test

The absence of human and E. coli DNA is confirmed by qPCR.

Prevention of PCR contamination

When assembling the amplification reactions, care should be taken to eliminate the possibility of contamination with undesired DNA.

Use separate clean areas for preparation of samples and reaction mixtures and for cycling.
Wear fresh gloves. Use sterile tubes and pipette tips with aerosol filters for PCR setup.
Use only water and reagents that are free of DNA and nucleases.
With every PCR setup, perform a contamination control reaction that does not include template DNA.

Typical use of dNTPs

0.2 mM of each nucleotide is the recommended final dNTP concentration in common PCR applications.
It is essential to have equal concentrations of all four dNTPs in the PCR mixture.
Every time before the use thaw and mix well each dNTP solution, especially highly concentrated ones.
To prepare on your own 1 ml dNTP mixtures of common 2 mM, 2.5 mM, 10 mM or 25 mM concentrations, follow the guidelines below:

1 ml Mix	100 mM dATP	100 mM dCTP	100 mM dGTP	100 mM dTTP	Water
2 mM dNTP Mix:	20 µl	20 µl	20 µl	20 µl	920 µl
2.5 mM dNTP Mix:	25 µl	25 µl	25 µl	25 µl	900 µl
10 mM dNTP Mix:	100 µl	100 µl	100 µl	100 µl	600 µl
25 mM dNTP Mix:	250 µl	250 µl	250 µl	250 µl	-

Use the ready dNTP mixes in PCR (at 0.2 mM final concentration each) according to the guidelines below:

Volumes of dNTP mixes for the indicated PCR volume
dNTP Mix concentration	Used for PCR as	50 µl PCR	25 µl PCR	20 µl PCR
2 mM	10×	5 µl	2.5 µl	2 µl
2.5 mM	12.5×	4 µl	2 µl	1.6 µl
10 mM	50×	1 µl	0.5 µl	0.4 µl
25 mM	125×	0.4 µl	0.2 µl	0.16 µl