Hot-Start PCR
biotechrabbit Hot-Start PCR products include highly purified YourTaq™ Hot-Start DNA Polymerase which is optimized for high yield of amplification of up to 5 kb DNA targets, even from low copy number. The enzyme shows excellent PCR specificity and sensitivity for a broad range of amplicons. YourTaq Polymerase is resistant to PCR inhibitors, such as blood (up to 20 %), ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors. PCR mixes combine YourTaq, optimized buffer and high-quality dNTPs for the most demanding PCR applications. In addition, the enzyme is suitable for amplification of GC-rich templates (up to 70 %) by using 5X PCR Enhancer.
Convenient — master mixes including a direct-load version make your job easier
Specific and sensitive — excellent performance for a broad range of amplicons
Efficient — abundant DNA even from low copy targets
Resistant — direct amplification from crude samples
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Hot-Start Taq DNA Polymerase, 5 U/µl
Features
- High PCR specificity and sensitivity
- Exceptionally pure Hot Start Taq DNA Polymerase for sensitive PCR applications and high yields
- Antibody-based Hot Start for fast polymerase activation
Learn More…Applications
- Hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- RT-PCR and TA cloning
Package Sizes
Starting at: €93.00Does not include applicable tax, shipping & handling, or other charges.
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2X Hot-Start PCR Master Mix
Features
- Highest PCR sensitivity without prolonged reactivation
- Optimized PCR Master Mix for minimal hands-on and fast setup
- Exceptionally pure Hot Start Taq DNA Polymerase and highest quality dNTPs
Learn More…Applications
- High-specificity and high-throughput hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- TA cloning
Package Sizes
Starting at: €114.00Does not include applicable tax, shipping & handling, or other charges.
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2X Hot-Start PCR Master Mix, lyophilized
Features
- Room-temperature stable enzymes and mixes
- Exceptionally pure Taq DNA Polymerase
- Hot-start for high PCR specificity and sensitivity
- Optimized Master Mix for fast setup
Learn More…Applications
- Ambient shipment and room-temperature storage
- Routine and demanding PCR applications
- PCR amplification up to 3 kb
- TA cloning
Package Sizes
Starting at: €149.00Does not include applicable tax, shipping & handling, or other charges.
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YourTaq™ Hot-Start DNA Polymerase, 5 U/µl
Features
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized buffer composition for increased yield of amplification
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- RT-PCR and TA cloning
Package Sizes
Starting at: €61.00Does not include applicable tax, shipping & handling, or other charges.
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2X YourTaq™ Hot-Start PCR Master Mix
Features
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized Master Mix for increased yield of amplification
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- TA cloning
Package Sizes
Starting at: €114.00Does not include applicable tax, shipping & handling, or other charges.
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2X YourTaq™ Hot-Start PCR Master Mix Direct-Load
Features
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized Master Mix for increased yield of amplification and direct loading on the gel
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- TA cloning
Package Sizes
Starting at: €120.00Does not include applicable tax, shipping & handling, or other charges.
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2X YourTaq™ Hot-Start PCR Master Mix, lyophilized
Features
- Stable enzyme and mix for ambient shipment and room-temperature storage
- Exceptionally pure YourTaq Hot Start DNA Polymerase
- Optimized Master Mix for increased yield of amplification
- Resistant to PCR inhibitor carry-over
- Excellent PCR specificity and sensitivity for a broad range of amplicons
Learn More…Applications
- Routine and demanding PCR amplification up to 3 kb
- Suitable for amplification of low target copy number
- TA cloning
Package Sizes
Starting at: €149.00Does not include applicable tax, shipping & handling, or other charges.
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ApStarTaq™ Hot-Start DNA Polymerase, 5 U/µl
Features
- Aptamer-based hot-start functionality
- No activation steps needed
- High PCR specificity and sensitivity
- Exceptionally pure Taq DNA Polymerase for sensitive PCR applications and high yields
Learn More…Applications
- Fast PCR reactions in endpoint and real-time analysis
- Hot-start PCR up to 3 kb
- Amplification of low-copy-number targets
- RT-PCR and TA cloning
Package Sizes
Starting at: €61.00Does not include applicable tax, shipping & handling, or other charges.
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UPstart™ Taq Antibody, 1 mg/ml
Features
- Inhibition of >95% Taq activity at 60°C
- 200 ng UPstart Taq Antibody are blocking 1 U Taq DNA polymerase
- Exceptionally pure— made in cell culture
Learn More…Applications
- Thermolabile inhibition of Taq DNA polymerases
- Convenient hot-start PCR setup at room temperature
- Fast polymerase activation with the first PCR denaturation step
Package Sizes
Starting at: €519.00Does not include applicable tax, shipping & handling, or other charges.
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UPstart™ Plus Taq Antibody, 1 mg/ml
Features
- Inhibition of >95% Taq activity at 60°C
- 200 ng UPstart Plus Taq Antibody are blocking 1 U Taq DNA polymerase
- Exceptionally pure— made in cell culture
Learn More…Applications
- Thermolabile inhibition of Taq DNA polymerases
- Convenient hot-start PCR setup at room temperature
- Fast polymerase activation with the first PCR denaturation step
Package Sizes
Starting at: €549.00Does not include applicable tax, shipping & handling, or other charges.
